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primary antibodies against spp1  (Wanleibio)

 
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    Wanleibio primary antibodies against spp1
    Primary Antibodies Against Spp1, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against spp1/product/Wanleibio
    Average 90 stars, based on 1 article reviews
    primary antibodies against spp1 - by Bioz Stars, 2026-03
    90/100 stars

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    Effect of LPS on endometrial receptivity genes in sheep. ( A ) The pro-conceptus elongation gene ISG15 , RSAD2 ( B ), and CXCL10 ( C ) on d12, d16, and d20 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( D ) The adhesion molecules ITGB1 , ITGB3 ( E ), ITGB5 ( F ), <t>SPP1</t> ( G ), and MUC1 ( H ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( I ) The endometrial receptivity markers HOXA10 , HOXA11 ( J ), and LIF ( K ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.
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    Effect of LPS on endometrial receptivity genes in sheep. ( A ) The pro-conceptus elongation gene ISG15 , RSAD2 ( B ), and CXCL10 ( C ) on d12, d16, and d20 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( D ) The adhesion molecules ITGB1 , ITGB3 ( E ), ITGB5 ( F ), SPP1 ( G ), and MUC1 ( H ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( I ) The endometrial receptivity markers HOXA10 , HOXA11 ( J ), and LIF ( K ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: LPS Disrupts Endometrial Receptivity by Inhibiting STAT1 Phosphorylation in Sheep

    doi: 10.3390/ijms252413673

    Figure Lengend Snippet: Effect of LPS on endometrial receptivity genes in sheep. ( A ) The pro-conceptus elongation gene ISG15 , RSAD2 ( B ), and CXCL10 ( C ) on d12, d16, and d20 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( D ) The adhesion molecules ITGB1 , ITGB3 ( E ), ITGB5 ( F ), SPP1 ( G ), and MUC1 ( H ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( I ) The endometrial receptivity markers HOXA10 , HOXA11 ( J ), and LIF ( K ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.

    Article Snippet: Subsequently, the permeabilized cells were blocked with 2% BSA for 1 h and incubated with primary antibodies against SPP1 (1:200; WL02378; Wanleibio Co., Ltd., Shenyang, China) and cytokeratin 18 (1:200; AF0191; Affinity Biosciences, Cincinnati, OH, USA) at 4 °C overnight.

    Techniques: Real-time Polymerase Chain Reaction

    Effect of LPS or fludarabine treatment on the expression of endometrial receptivity-related genes under hormone treatment. ( A ) The protein level of p-STAT1 and T-STAT1 in sEECs. ( B ) The secretion of PGE2 and PGF2α in sEECs. ( C – E ) The pro-conceptus elongation genes ISG15 , RSAD2 , CXCL10 , adhesion molecules ITGB1/3/5 , MUC1 , SPP1 , and receptivity markers HOXA10 , HOXA11 , LIF mRNA expression levels in sEECs. GAPDH (sheep) was used as the reference gene in all samples. ( F ) Confocal microscope images of SPP1 expression in four treatment groups. Red: Cy3-labeled SPP1 protein; blue, DAPI-labeled nuclei; scale bar: 20 µm. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: LPS Disrupts Endometrial Receptivity by Inhibiting STAT1 Phosphorylation in Sheep

    doi: 10.3390/ijms252413673

    Figure Lengend Snippet: Effect of LPS or fludarabine treatment on the expression of endometrial receptivity-related genes under hormone treatment. ( A ) The protein level of p-STAT1 and T-STAT1 in sEECs. ( B ) The secretion of PGE2 and PGF2α in sEECs. ( C – E ) The pro-conceptus elongation genes ISG15 , RSAD2 , CXCL10 , adhesion molecules ITGB1/3/5 , MUC1 , SPP1 , and receptivity markers HOXA10 , HOXA11 , LIF mRNA expression levels in sEECs. GAPDH (sheep) was used as the reference gene in all samples. ( F ) Confocal microscope images of SPP1 expression in four treatment groups. Red: Cy3-labeled SPP1 protein; blue, DAPI-labeled nuclei; scale bar: 20 µm. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.

    Article Snippet: Subsequently, the permeabilized cells were blocked with 2% BSA for 1 h and incubated with primary antibodies against SPP1 (1:200; WL02378; Wanleibio Co., Ltd., Shenyang, China) and cytokeratin 18 (1:200; AF0191; Affinity Biosciences, Cincinnati, OH, USA) at 4 °C overnight.

    Techniques: Expressing, Microscopy, Labeling